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. 2020 May 19;8:366. doi: 10.3389/fcell.2020.00366

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Copyright © 2020 Yin, Song, Zhao, Liu, Kang, Lee, Kim, Zhang, Shim, Liu, Dong and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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KRT18 is potential target of ESCC. (A) Knocking-down the expression of KRT18 in KYSE30 cell was detected by Western blotting. (B) Anchorage-independent cell growth was decreased after treated with xanthohumol in mock transfected cells or KRT18 knockdown cells but the colonies number did not further decrease after treated with xanthohumol in the KRT18 knockdown cells. (C) Representative photographs of the colonies. (D) KRT18 gene was overexpressed in HEK293T cells. Full length of KRT18 gene was transfect into HEK293T cells with simple-fect transfection reagent. Then it was confirmed the expression by Western blotting. (E) Overexpression of KRT18 promoted the colony formation in anchorage-independent cell growth, and was more sensitive to xanthohumol treatment compared to mock transfected. (F) Representative photographs of the colonies formed in soft agar. The asterisks (*p < 0.05, **p < 0.01, ***p < 0.001) indicated a significant difference between untreated control and xanthohumol-treated cells. Data were shown as means ± SD of values from triplicate samples and similar results were obtained from three independent experiments.