TABLE 1.

Assays for ROS and antioxidant agents.

Assay	Method	Mechanism	Detection
General ROS	Dihydrorhodamine 123 and CellROX dyes	Dyes fluoresce green upon oxidation	Fluorescence
Hydrogen peroxide	Amplex red assay (Amplex red reagent + horseradish peroxidase)	Generation of fluorescent resorufin	Absorbance/fluorescence
Superoxide	MitoSOX dye	Dye oxidized by superoxide in mitochondria	Fluorescence
Superoxide dismutase	Colorimetric immunoassay	Generation of a yellow product	Absorbance
Peroxidase	Amplex red assay (Amplex red reagent + hydrogen peroxide)	Generation of fluorescent resorufin	Absorbance/fluorescence
Catalase	Colorimetric assay	Coupling of quinoneimine dye with H2O2	Absorbance
Glutathione	Colorimetric assay	Generation of a colored compound by a reaction of chromogen with glutathione	Absorbance
Ascorbic acid	Colorimetric assay	Ferric reducing/antioxidant ascorbic acid (FRASC) chemistry	Absorbance