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. 2020 May 19;8:463. doi: 10.3389/fbioe.2020.00463

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Copyright © 2020 Zheng, Tian, Gao, Yu, Xia, Feng, Dong, Wu and Sui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface topography influences cell adhesion, proliferation and migration. (A) SEM observation of pseudopodia of cell extending on S, SLA, and SLM-AHT titanium surfaces after 6 h of seeding. (B,C) Immunofluorescence images of cell (red, F-actin; blue, DAPI) on S, SLA and SLM-AHT titanium surfaces after 6 and 24 h of seeding. (D) Cell count on S, SLA and SLM-AHT titanium surfaces for 6, 24, and 72 h. (E) Scratch assay of cell on different groups culturing for 0, 6, and 24 h visualized via DAPI (blue) staining. (F) Relative closure determined by measuring wound widths from images (E) in the upper panel.