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. 2020 May 19;8:463. doi: 10.3389/fbioe.2020.00463

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Copyright © 2020 Zheng, Tian, Gao, Yu, Xia, Feng, Dong, Wu and Sui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Integrin α2 influences cell osteogenic differentiation. (A) The relative mRNA levels of Runx2, Col1a1, and Ocn in scramble and integrin α2 silenced cell. (B) Western blot analysis of Runx2, Col1a1, and Ocn proteins in scramble and integrin α2 silenced cell. (C) Immunofluorescence staining showing integrin α2 dependent diverse expression of Runx2 (red, F-actin; blue, DAPI: green, Runx2). (D) The relative mRNA levels of Runx2, Col1a1, and Ocn in scramble and integrin α2 silenced cell culturing on S, SLA, and SLM-AHT surfaces for 3 days. Statistical significance was determined by one-way ANOVA. Error bars represent SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.005.