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. 2020 May 19;11:800. doi: 10.3389/fimmu.2020.00800

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Copyright © 2020 Bujor, El Adili, Parvez, Marden and Trojanowska.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Monocytes isolated from SSc patients have low levels of Fli1. (A) Monocytes, T and B lymphocytes were isolated from healthy controls (HC) and SSc patients (diffuse-dcSSc or limited-lcSSc) and mRNA levels of Fli1 were analyzed using quantitative RT-PCR. One way ANOVA (GraphPad Prims 7) was used to compare SSc to control. (B) Isolated peripheral blood mononuclear cells (PBMCs) from SSc and healthy controls (HC) were stained with CD14 for monocytes (red) and Fli1 (green) antibodies, and DAPI was used to counterstain the nuclei. Representative images and quantification are shown (n = 3 each). **p < 0.01.