FIGURE 4.

Fli1 downregulation in myeloid cells does not induce TGFβ pathway. (A) THP1 cells were transfected as described with scr and Fli1 siRNA and levels of TGFβ isoforms 1, 2, and 3 were measured at the mRNA levels 48 h later using RT-PCR. n > 3 each, not statistically significant. (B) THP1 monocytes were transfected with siRNA and 48 h later they were plated directly over confluent, serum starved human dermal fibroblasts. After 24 and 48 h, cells were washed and cell layers collected and protein extracted. Levels of P-Smad2 (Ser465/467) and total Smad2/3 were assessed by Western Blot, with TGFβ as positive control.