Reagent or resource	Source	Identifier
BACTERIAL STRAINS
E. coli BL21DE3 cells	Gift from Dr. M. Seeliger (SUNY, Stony Brook)	N/A
Src kinase purification, assays, and crystal structure
pET28a Src kinase expression vector	Gift from Dr. M. Seeliger (SUNY, Stony Brook)	N/A
Custom oligos:AAGCTGGGGCAGGGCAGCTTTGGAGAGGTCTGG	Sigma-Aldrich	N/A
QuikChange II Site-Directed Mutagenesis Kit	Agilent Technologies	Cat # 200524
Kanamycin	Sigma-Aldrich	Cat # K1377
Streptomycin	Sigma-Aldrich	Cat # S9137
Chloramphenicol	Sigma-Aldrich	Cat # C0378
Bosutinib	Sigma-Aldrich	Cat # PZ0192
SM1-71	Prof. Nathanael Gray, DFCI, Harvard	https://graylab.dana-farber.org/
DGY-06-116	Prof. Nathanael Gray, DFCI, Harvard	https://graylab.dana-farber.org/
NJH-01-111	Prof. Nathanael Gray, DFCI, Harvard	https://graylab.dana-farber.org/
LabChip® EZ Reader automated microcapillary electrophoresis platform	PerkinElmer	https://www.perkinelmer.com/product/assy-ez-reader-ii-ship-level-122919
Fluorescently labeled peptide-4 substrate	Perkin Elmer	https://www.perkinelmer.com/product/dkp-peptide-4-packed-760348
EZ-Link™ Sulfo-NHS-Biotinylation Kit	ThermoFisher	Cat # 21425
Biacore S200	GE Healthcare	https://www.gelifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-s200-p-05541
Biotin Capture Kit and Sensor Chip CAP	GE Healthcare	Cat # 28920233
Deposited data
Src-DGY-06-116	https://www.rcsb.org (this study)	PDB ID: 6E6E
SOFTWARE AND ALGORITHMS
EZReader 3.0 software	Perkin Elmer	https://www.perkinelmer.com/lab-products-and-services/resources/software-downloads.html
GraphPad software version 7	GraphPad Software	www.graphpad.com
HKL3000	(Minor et al., 2006)	https://sbgrid.org/
PHENIX 1.14rc3_319	(Adams et al., 2010)	https://sbgrid.org/
Coot	Emsley and Cowtan, 2004	https://sbgrid.org/
CCP4 7.0	Winn et al., 2011	https://sbgrid.org/
Pymol 2.3	Schrodinger, LLC	https://sbgrid.org/
LIGPLOT	Laskowski R A, Swindells M B (2011)	https://www.ebi.ac.uk/thornton-srv/software/LigPlus/
Biacore™ insight evaluation software	GE Healthcare Life Sciences, USA.	https://www.gelifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-s200-p-05541