TABLE 3.

Comparison of NSC-derived hMO models: Overview of selected features of hMOs approaches published in Monzel et al., 2017; Smits et al., 2019b.

	Monzel et al. (2017)	Smits et al. (2019b)
Culture conditions
Used cell type	smNPCs	mfNPCs
Number of cells	9,000	3,000
Embedding	Yes	No
Agitation	Yes	No
mDANs
TH + cells*	∼64% (d61)	∼54% (d70)
Regionalization	Yes	No
A9/A10 specificity	Yes	Yes
DAT	Yes (d61)	Yes (d70)
D2/D3 receptor responsive	Yes	Yes
DA release	No	Yes
NM	Yes (>d149)	Yes (>d100)
Other cell types
Oligodendrocytes	Yes (d61)	n/a
Astrocytes	Yes (d61)	n/a
Neuronal subtypes	n/a	Yes

Neuronal subtypes refers to the analysis whether neurons other than dopaminergic neuros are determined. As starting cell population, small molecule neural precursor cells (smNPCs) are used in Monzel et al., 2017, while Smits et al. (2019b) starts with midbrain floor plate neural progenitor cells (mfNPCs). *, different methods applied to determine and calculate TH content.