Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 19;8:357. doi: 10.3389/fcell.2020.00357

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Gupta, Viswanatha and Patel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Chromosome 14 ideogram showing genomic location of the IGH gene region (red box). The right side diagram schematically illustrates the germline configuration of V, D, and J IGH genes (top) and the rearranged IGH gene in a given B-cell. Remarkable IGH repertoire diversity is generated by the rearrangement potential of many V, D, and J genes, as well as the effects of junctional nucleotide alterations (trimming and additions of random nucleotide pool bases by the enzyme terminal deoxynucleotidyl transferase, TdT) (A). IGH Rearrangement and Junctional Sequences. Leader primers typically provide a longer sequence as compared to the framework primers. Evaluation of junctional sequences including the Cys104 and Trp118 anchors is essential for SHM determination (B).