Fig. 4.

Effect of CMF size on cell density of the fabricated 3D tissues. (a) Schematic illustration of the fabrication of 3D tissues with CMFs by sedimentary culture. (b) Toluidine blue (TB) images of the obtained 3D tissues using uCMF-200, CMF-200, and CMF-20, respectively. The bottoms are magnified images of the uppers. (c) The quantitative comparison of cell density in TB images and ECM area in HE images using CMFs. The asterisks denote statistically significant differences using a two-sample Student's t-test (∗p<0.01) for each comparison (n = 5). The asterisk of CMF-20 for cell density denotes statistically significant difference to both uCMF-200 and CMF-200. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)