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. 2020 Apr 21;22(1):483–493. doi: 10.3892/mmr.2020.11085

Figure 1.

Figure 1.

miR-93 attenuates H/R-induced cell injury and cell apoptosis in HUVECs. (A) Time course of miR-93 expression in HUVECs before and after H/R was examined using RT-qPCR. n=4-5 replicates per group. (B) RT-qPCR analysis revealed an increased expression of miR-93 in HUVECs transfected with miR-93 mimic. n=4–5 replicates per group. After 48 h of transfection, HUVECs were exposed to 4 h hypoxia, followed by 24 h reoxygenation. (C) Transfection of HUVECs with miR-93 mimic decreased LDH activity and (D) increased cell viability following H/R injury. n=4 replicates per group. (E) Representative bands of (F) cleaved caspase-3, (G) Bax and (H) Bcl2. Transfection of HUVECs with miR-93 mimic decreased the expression levels of cleaved caspase-3 and Bax, and increased the expression of Bcl2 after H/R injury. n=4–5 replicates per group. Data are presented as the mean ± SEM. Each experiment was repeated three times. *P<0.05 vs. the indicated group. H/R, hypoxia/reoxygenation; HUVECs, human umbilical vein endothelial cells; scr, scrambled; miR, microRNA; LDH, lactate dehydrogenase; RT-qPCR, reverse transcription-quantitative PCR.