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. 2019 Oct 9;42(2):150–158. doi: 10.1016/j.htct.2019.06.007

Figure 1.

Figure 1

Establishment of improved conditions for lentiviral production. Effect of plasmid ratio on viral production: (A) Viral titer (TU/mL) using different plasmid ratios (transgene:gag-pol:VSV-G:rev). Data presented as mean ± SD, n = 2. Non-significant difference (Mann-Whitney U-test). (B) Effect of viral supernatant harvesting times on Viral titer (TU/mL). Single harvests at 24 and 48 h post-transfection (hpt), and two harvests at 24 and 48 hpt. Data presented as mean ± SD, n = 3. **P < 0.002 (ANOVA followed by Holm-Sidak). (C) Effect of transfection agent on Viral titer (TU/mL). Polyethyleneimine (PEI) and Lipofectamine® 2000 (Lipofectamine) were employed in the absence or presence of NaBu (5 mM). Data presented as mean ± SD, number of replicates indicated by number of points, *P < 0.05; **P < 0.002; ***P < 0.0002; ****P < 0.0001 (ANOVA followed by Holm-Sidak). (D) Effect of concentration methods on lentiviral recovery. Ultracentrifugation (UC), tangential flow filtration (QuixStand - QS), tangential flow filtration, followed by ultracentrifugation (QS + UC), ultrafiltration by membrane centrifugation (VivaSpin - vs) were employed. Data presented as mean ± standard deviation, number of replicates indicated by the number of points, **P < 0.002; ****P < 0.0001 (ANOVA followed by Holm-Sidak).