Table I.
Primers and universal TaqMan probes used for the reverse transcription-quantitative PCR.
| Gene numbera | NCBI reference sequence | Primer sequence (5′→3′) | Probe |
|---|---|---|---|
| Dynein cytoplasmic 1 light intermediate chain 1 | NM_016141 | F: CTGGTGTGAGTGGTGGTAGC | 10 |
| R: TCTGCATGAACATCTAAGACAGG | |||
| Mucin 2 | NM_002457 | F: ATGCCAGCATTTGCATCC | 89 |
| R: GGCACCCTGGTCTCATTG | |||
| GAPDH | NM_002046 | F: CTCTGCTCCTCCTGTTCGAC | 60 |
| R: ACGACCAAATCCGTTGACTC |
a
Probe number from the Human Universal Library of Roche Diagnostics GmbH. F, forward; R, reverse.