Figure 2.
Role of NMN in Dex-induced osteogenic inhibition of BMSCs. BMSCs were exposed to 10−5 M Dex and NMN (1, 5 and 10 mM). (A) Reverse transcription-quantitative PCR was used to detect Runx2, ALP and OCN mRNA expression in BMSCs. (B) Western blotting was used to determine the Runx2 and ALP protein expression levels in BMSCs. (C) Alizarin red and ALP staining assays were used to assess the osteogenic function of BMSCs treated with 10−5 M Dex for 7 days. Scale bar=20 µm. All experiments were performed ≥3 times. P<0.05 was considered to indicate a statistically significant difference. ***P<0.001 and **P<0.01, as indicated. NMN, nicotinamide mononucleotide; Dex, dexamethasone; BMSC, bone mesenchymal stem cells; ALP, alkaline phosphatase; Runx2, Runt-related transcription factor 2; OCN, osteocalcin.