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. 2020 Apr 24;22(1):257–264. doi: 10.3892/mmr.2020.11094

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — mROS mediates Ti ion induced autophagy in osteoblastic cells. (A) Quantification of mROS levels using a fluorescence spectrometer after osteoblastic cells were treated with TiCl₄. Osteoblastic cells were preincubated with 10 mM Mito-TEMPO for 2 h and treated with 50 µM TiCl₄, then the(B) mROS levels, (C) LC3 protein expression level and (D) and cell viability were determined. Data are presented as a percentage of the control group, which was set at 100%. *P<0.05 vs. control group, ^#P<0.05 vs. TiCl₄ group. Ti, titanium;TiCl₄, Ti tetrachloride; LC3, microtubule associated protein 1 light chain 3 α; mROS, mitochondrion-derived reactive oxygen species.