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. 2020 Apr 24;22(1):257–264. doi: 10.3892/mmr.2020.11094

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Ti ions can increase acetylated-SOD2 expression level, and decrease SIRT3 protein expression level and activity in a dose-dependent manner. (A) SOD2 activity in osteoblastic cells exposed to TiCl₄. (B) Representative immunoblot of SOD2 and SIRT3 protein expression levels in osteoblastic cells. GAPDH was used as an internal standard. (C) Acetylation of SOD2 after TiCl₄ exposure was determined by immunoprecipitation with an anti-SOD2 antibody, followed by immunoblot analysis of acetylated-lysine. GAPDH used as an internal standard for protein loading. (D) Reverse transcription-quantitative PCR was used to detect SIRT3 mRNA expression levels. (E) SIRT3 activity level. Data are presented as a percentage of the control group, which is set at 100%. *P<0.05 vs. control group. Ti, titanium; SOD2, superoxide dismutase 2; SIRT3, sirtuin 3; TiCl₄, Ti tetrachloride.