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Primers used for reverse transcription-quantitative PCR.
| Gene | Primer sequence (5′→3′) |
|---|---|
| GAPDH | F: GCACCGTCAAGGCTGAGAAC |
| R: TGGTGAAGACGCCAGTGGA | |
| ALP | F: GCTCATGCATAACATCAGGGACA |
| R: TCGTCACTCTCATACTCCACATCAG | |
| RUNX2 | F: CACTGGCGCTGCAACAAGA |
| R: CATTCCGGAGCTCAGCAGAATAA | |
| U6 | F: GCTTCGGCAGCACATATACTAAAAT |
| R: CGCTTCACGAATTTGCGTGTCAT |
F, forward; R, reverse; ALP, alkaline phosphatase; miR, microRNA; RUNX2, runt-related transcription factor 2.
