Figure 10.

PhenT (1 mg/kg, 24 h prior to MCAo) decreased neuronal cell apoptosis/PNCD in the cortex induced by transient (60 min) MCAo in rat: If a candidate drug mitigates PNCD in one animal model of brain injury (eg, PhenT in mTBI and modTBI), in theory it should do the same across animal models of brain injury. To test this notion, PhenT was evaluated in a classical model of cerebral anoxia (MCAo in rat).26 (A) Representative immunohistochemistry demonstrating TUNEL staining and NeuN. Quantitative evaluation of (B) NeuN (neuronal marker) + cells, (C) TUNEL + cells, (D) TUNEL/NeuN cell ratio across similar size fields in penumbra of MCAo animals, (E) biochemical analysis of key PNCD proteins (N = 3/ group, means ± SEM, * comparison to control vehicle: *P ≤ .05, **P ≤ .01 and ***P ≤ .001; ^# comparison MCAo vehicle to MCAO PhenT: ^## P ≤ .01).26 Across measures, PhenT mitigated ischemia‐induced neuronal cell loss, TUNEL staining and changes in PNCD‐related proteins