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. 2020 May 19;7:256. doi: 10.3389/fvets.2020.00256

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Copyright © 2020 Wallace, Mather, Kara, Naicker, Mokoena, Pretorius, Nefefe, Thema and Babiuk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic analysis of memory T cells in PBMCs collected from calves. (A) CD4+ CD45RO+ cells and (B) CD8+ CD45RO+ cells in Group 2 (vaccinated and LSDV challenged animals) when PBMCs were stimulated with LSDV. Averages for the Group 1 (mock-vaccinated control animals: D1-1 to D1-5) are depicted in the line graph in both panels. PBMCs stimulated with antigen were normalized against unstimulated PBMCs. Significant differences, as compared to the negative control group with P ≤ 0.01, are indicated with**.