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. 2020 May 19;7:256. doi: 10.3389/fvets.2020.00256

Table 1.

Calf identity numbers, group numbers, the inoculum used for both inoculation and challenge and their relevant titres and routes and day of challenge.

Animal number Group Inoculum (titer) Day of challenge and challenge virus Titer
D1-1 1 diluent*
(2.0 ml, SC)
Day 21 post-inoculation.
LSDV Warmbaths isolate
5 × 104 TCID50/ml (2.0 ml, SC)
&
5 × 105 TCID50/ml (2.0 ml, IV)
D1-2
D1-3
D1-4
D1-5
D2-1 diluent*
(2.0 ml, SC)
Day 28 post-inoculation.
RVFV strain M35/74
3.0 × 106 TCID50, 1.0 ml IV
D2-2
D2-3
D2-4
D2-5
A1-1 2 LSD-RVF.mf
(1,0 × 103 TCID50)
Day 21 post-inoculation.
LSDV Warmbaths isolate
5 × 104 TCID50/ml (2.0 ml, SC)
&
5 × 105 TCID50/ml (2.0 ml, IV)
A1-2
A1-3
A1-4
A1-5
C2-1 LSD-RVF.mf
(1,0 × 103 TCID50)
Day 28 post-inoculation.
RVFV strain M35/74
3.0 × 106 TCID50, 1.0 ml, IV
C2-2
C2-3
C2-4
C2-5
*

2.0 ml of vaccine diluent, obtained from Onderstepoort Biological products.