Table 1.
Animal number | Group | Inoculum (titer) | Day of challenge and challenge virus | Titer |
---|---|---|---|---|
D1-1 | 1 | diluent* (2.0 ml, SC) |
Day 21 post-inoculation. LSDV Warmbaths isolate |
5 × 104 TCID50/ml (2.0 ml, SC) & 5 × 105 TCID50/ml (2.0 ml, IV) |
D1-2 | ||||
D1-3 | ||||
D1-4 | ||||
D1-5 | ||||
D2-1 | diluent* (2.0 ml, SC) |
Day 28 post-inoculation. RVFV strain M35/74 |
3.0 × 106 TCID50, 1.0 ml IV | |
D2-2 | ||||
D2-3 | ||||
D2-4 | ||||
D2-5 | ||||
A1-1 | 2 | LSD-RVF.mf (1,0 × 103 TCID50) |
Day 21 post-inoculation. LSDV Warmbaths isolate |
5 × 104 TCID50/ml (2.0 ml, SC) & 5 × 105 TCID50/ml (2.0 ml, IV) |
A1-2 | ||||
A1-3 | ||||
A1-4 | ||||
A1-5 | ||||
C2-1 | LSD-RVF.mf (1,0 × 103 TCID50) |
Day 28 post-inoculation. RVFV strain M35/74 |
3.0 × 106 TCID50, 1.0 ml, IV | |
C2-2 | ||||
C2-3 | ||||
C2-4 | ||||
C2-5 |
2.0 ml of vaccine diluent, obtained from Onderstepoort Biological products.