Table 1.
Range of parameters tested for six protein assays on paper platform.
| Parameters | Biuret | Lowry | Bradford | BCA | BCG | TBPB | |
|---|---|---|---|---|---|---|---|
| Substrate type | Cellulose | Cellulose | Microfiber | Cellulose | Cellulose | Cellulose | |
| Reagent volume (μL) | range tested | 3–9 | 3–9 | 5–10 | 6–10 | 3 | 2–3 |
| selected | 9 | 3 | 10 | 10 | 3 | 3 | |
| Reagent concentration | range tested | 0.15%–3.0% | 1%–35% | 0.01%–0.5% | 1%–2.5% | 30–150 mM | 0.5–10 mM |
| selected | 0.75% | 2% | 0.01% | 1.5% | 120 mM | 3 mM | |
| Assay time (minute) | range | 0–50 | 0–126 | 0–20 | 0–30 | 0–22 | 0–50 |
| selected | 12 | 5 | 14 | 8 | 16 | 5 | |
| RGB color space | Green | Red | Red | Green | Red | Red | |
| Order of fluid addition | Sample first | Reagent first | Reagent first | Sample first | Reagent first | Reagent first |