Table 2.
Analytical figures of merits of six protein assays on paper platform.
| Biuret | Lowry | BCA | Bradford | BCG | TBPB | |
|---|---|---|---|---|---|---|
| Protein tested (mg/mL) | 5.0–120.0 | 0.05–15.0 | 0.097–100.0 | 0.05–4.0 | 10.0–100.0 | 0.5–16.0 |
| Linear range (mg/mL) | 10.0–110.0 | 0.1–12.0 | 3.1–25.0 | 0.1–3.0 | 10–50 | 0.5–6 |
| r2 | 0.960 | 0.976 | 0.954 | 0.967 | 0.980 | 0.958 |
| Slope | 0.04 | 2.81 | 1.16 | 8.43 | 1.84 | 12.07 |
| Precision (%) | 3.7 | 1.6 | 1.2 | 3.1 | 2.9 | 6.4 |
| Accuracy (%) | 92.2 ± 11.9 | 91.4 ± 15.0 | 102.4 ± 18.9 | 94.1 ± 6.4 | 91.5 ± 8.8 | 73.3 ± 28.4 |
| LOD (mg/mL) | NA | 2.2 | 0.3 | 0.5 | 3.8 | 0.9 |
| LOQ (mg/mL) | NA | 7.3 | 1.2 | 1.5 | 12.8 | 2.9 |