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. 2020 May 3;56:102795. doi: 10.1016/j.ebiom.2020.102795

Fig. 5.

Fig 5

SPOP promote the proliferation and invasion of kidney cancer cells. (a) The CTG assay was used to detect the proliferation capability of A498 kidney cancer cells infected with SPOP lentiviral shRNA. Data are shown as mean ±SD of three independent experiments. *P<0.05. (b) The CTG assay was used to detect the proliferation capability of A498 and 786-O kidney cancer cells transfected with SPOP plasmids. Data are shown as mean ±SD of three independent experiments. *P<0.05. (c) Transwell chambers assay was performed to detect the invasion capability of 786-O kidney cancer cells transfected with the indicated plasmids. (d) The quantification of invasion cells in (c). Data are shown as mean ±SD of three independent experiments. **P<0.01. (e) Transwell chambers assay was performed to detect the invasion capability of 786-O kidney cancer cells infected with the indicated lentiviral shRNA or transfected with the indicated siRNA. (f) The quantification of invasion cells in (e). Data are shown as mean ±SD of three independent experiments. *P<0.05, **P<0.01. (g) Transwell chambers assay was performed to detect the invasion capability of 786-O kidney cancer cells transfected with the indicated plasmids. (h) The quantification of invasion cells in (g). Data are shown as mean ±SD of three independent experiments. *P<0.05, **P<0.01. ns: no significant difference.