Fig. 1.

CD24 is highly expressed in ovarian cancer cell lines and primary ovarian cancer cells, and can be detected by CD24-AF750 NIR staining. (a) Flow cytometry analysis of EOC cell lines OV-90, Caov-3, COV318 and Skov-3 stained with the monoclonal antibody CD24 conjugated to AF750 (n = 3). Each column represents the mean with SD. (b) CD24 expression was evaluated by flow cytometry, and antigen-binding capacity was quantified for OV-90 compared to monoclonal IgG1 antibody (mAb) control. (c) Validation of target-specific binding. White light (colour) image, near infrared (NIR 800) fluorescent image and pseudo-coloured fluorescence intensity merge image of CD24-AF750 stained EOC cell pellets (2•5 × 10⁷ tumour cells, scale bar = 1 cm). (d) Immunohistochemistry of four different patient-derived xenograft (PDX) ovarian tumours, stained with anti-CD24. Immunoreactivity score (IRS) 4•25 ± 0•96 (scale: 1ow = 1 – 7 = high). Images were captured at 100× and 200× magnification (scale bar = 25 μm).