Fig. 4.
Survival surgery in OV-90luc+ orthoptic xenografts. (a) Intraoperative white light (colour), near infrared (NIR 800) fluorescent and pseudo-coloured fluorescence intensity merge images from CD24-targeted fluorescence image-guided surgery (FIGS) of the primary ovarian tumour and intra-abdominal metastases. Ex vivo bioluminescence imaging was performed on resected lesions to confirm tumour specificity. (Scale bars = 1 cm). (b) Comparison of the number and total weight of resected metastatic lesions between the CD24 FIGS cohort (n = 8) and white light control surgery (n = 8). (c) Comparison of post-operative bioluminescence signal intensity between CD24-FIGS resected mice (n = 6) and white light control mice (n = 6). Longitudinal whole-body bioluminescence imaging was performed weekly for four weeks post-surgery and the bioluminescent signal was normalised to the presurgical bioluminescence signal of each individual mouse. (d) Mice were allocated a disease stage based on pre-surgical bioluminescence signal and intraoperative disease dissemination (e) Comparison of (normalised) pre- and post-surgical bioluminescence signal between CD24-FIGS and white light surgery in Stage I (f) Stage II and (g) Stage III resected mice (p > 0•05). Statistical analysis (Mann–Whitney U test) with p < 0•05 (*) were regarded as statistically significant.