Figure 3.

Antioxidant effect of the WSE evaluated using DCFH-DA (2ʹ,7ʹ-Dichlorofluorescin Diacetate) assay on A549 (A), T47D-KBluc (B), MCF-7(C) and HGF (D). The cellular model was pre-exposed to the extract (25, 40, and 50 µg/mL) or N-acetylcysteine (NAC) (20 mM) for 24 h, and further incubated with 50 µM DCFH-DA. The antioxidant effect of the WSE was evaluated after 2 h in stimulated (250 µM H₂O₂) and un-stimulated conditions. The results are expressed as relative means ± standard deviations (six technical replicates for each of the three biological replicates) where the negative control (DMSO 0.2%) is 100%. The asterisks (*) indicate significant differences compared to the positive control (250 µM H₂O₂) in stimulated conditions, while (#) indicate significant differences compared to the negative control in non-stimulated conditions (ANOVA + Tukey; p < 0.05).