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Experimental schedule of a TMA-induced AD-like mouse model. After sensitization of BALB/c mice by exposing them to 5% TMA (50 μL) on the shaved flank on Day 0, an AD-like symptom was induced on the ears of the mice by exposing them to 2% TMA (20 μL) on Days 5 to 14 daily. Piperine (2 and 4 mg/mL) or dexamethasone (10 μg/mL) was topically applied on Days 5 to 14 daily 1 h before TMA exposure.
