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. 2020 May 8;25(9):2220. doi: 10.3390/molecules25092220

Table 2.

Cytotoxic IC50 values of HAA2020, the eleven inhibitors and their combinations in HL60 cells (24 h, nM).

No. Inhibitor Targets IC50 nM IC50 (nM) Combination HAA2020/Inhibitor a (50:1) CI b at Fa = 0.9
1 PHA-767491 CDK1/2,CDK5, Cdc7/CDK9, GSK3-β,MK2 PLK1, CHK2 26.1 ± 3.8 21.8 ± 3.2 0.991
2 Dinaciclib CDK1, CDK2, CDK5, CDK9 8.2 ± 0.3 4.9 ± 1.3 0.628
3 ON123300 CDK4, Ark5, PDGFRβ, FGFR RET, Fyn 37.3 ± 3.5 1872.5 ± 45.9 >1
4 AMG 925 CDK4/FLT3 54.4 ± 7.7 3355 ± 347.8 >1
5 KW2449 FLT3, EGFR, FGFR1, Bcr-Abl PDGFRβ, IGF-1R 56.5 ± 10.8 2535.5 ± 318.9 >1
6 AZD2932 FLT3, c-Kit, VEGFR, PDGFRβ 15.2 ± 3.1 2394 ± 380.4 >1
7 Dasatinib c-Kit, Abl, Src 12.1 ± 2.9 3036 ± 181.1 >1
8 BGJ398 Kit, FGFR, VEGFR2, Abl, Fyn, Lck, Lyn, Yes 16.2 ± 1.7 2952.5 ± 170.6 >1
9 Saracatinib EGFR, Src, Yes, Fyn, Lyn, Blk, Fgr, Lck, Abl 16.8 ± 1.2 1821.5 ± 40.3 >1
10 Lapatinib EGFR, HER2 12.1 ± 2.0 2383.5 ± 245.3 >1
11 Taselisib PI3Kα/δ/γ 7.9 ± 0.4 2647 ± 203.6 >1
HAA 2020 VEGFR2, EGFR, HER2 1814.5 ± 230.8 - -

a: inhibitors 1–11. b: Combination index. (-): not applicable. Data are represented as mean ± SD (n = 3). Experiment was repeated 3×. IC50 of some combinations is more than 100 nM because the IC50 of HAA2020 alone is more than 100 nM.