Table 2.
Peptide Br-p sequence fragments assigned in RapidDeNovo sequencing; theoretical activity character and pI.
| Sequence Fragment Identify by Rapid de Novo | m/z | S/N * | Asteraceae Family Proteins Possible Source of Peptides ** |
Probable Peptide Sequence | Activities *** | Theoretical pI **** |
|---|---|---|---|---|---|---|
| VQGR | 2470 | 24.2 | photosystem I P700 chlorophyll a apoprotein A1 (Arctium lappa) | QPRALSIVQGRAVGVTHYLLGGIA | Antioxidant: YLL Activating ubiquitin-mediated proteolysis: RA |
10.84 |
| WHM | 2696 | 388.2 | ribulose-1.5-bisphosphate carboxylase/oxygenase large subunit. partial (Arctium eriophorum) | FTQDWVSLPGVLGHPWGNAWHMPA | Antioxidant: PWG, AW, WG | 5.97 |
| GAAV | 2700 | 1180.0 | ribulose 1-5 bisphosphate carboxylase oxygenase. partial (Arctium lappa) | QPGVPPEEAGAAVAAESSTGTWTTVWT | Antioxidant: TW, VW, GAA, GTW | 3.67 |
| WFANH | 2714 | 73.4 | histone-binding protein RBBP4 (Artemisia annua) | YDWFANHNLLWPSLSCRWGPLE | Antioxidant: RW, LW | 5.32 |
| TLAW | 2718 | 55.9 | photosystem I P700 chlorophyll a apoprotein A2 (Arctium lappa) | WRGYWQELIETLAWAHERTPLA | Antioxidant: AH, EL, AW | 5.50 |
| RFFASKS | 2720 | 35.1 | fructan:fructan 1-fructosyltransferase (Arctium lappa) | LRCDYGRFFASKSLYDPLKKRR | Immunomodulating: YG Antioxidant: LY, LK Bacterial permease ligand: KK |
10.18 |
| STAG | 2737 | 36.4 | NADH dehydrogenase subunit F. partial (Arctium lappa) | SWLYSPIFAIIAWSTAGLTAFYMC | Antioxidative LY, AW |
5.24 |
| EYPTGR | Acyl-CoA dehydrogenase. conserved site-containing protein (Cynara cardunculus var. scolymus) |
IQCLGGNGYM NEYPTGRYLR DAKL | Antioxidative: QCL Alpha-glucosidase inhibitor: YP |
8.15 | ||
| WEYPT | transposase. MuDR. MULE transposase domain protein (Artemisia annua) | AEAVNFVGEYHEWEYPTHIKPII | Antioxidative: KP alpha-glucosidase inhibitor: YP, EA |
4.87 | ||
| PFFY | 2783 | 27.2 | NADH dehydrogenase subunit 7 (Arctium lappa) | LWLGPFMADIGAQTPFFYIFRER | Antioxidative: LW | 6.07 |
| NWFKPG | 3034 | 163.9 | Resistance protein candidate. partial (Lactuca sativa) | TLLVLDDVDHIDQLEALAGDLNWFKPG | Antioxidative: KP Alpha-glucosidase inhibitor: EA Activating ubiquitin-mediated proteolysis: LA |
3.90 |
| nematode resistance-like protein. partial (Artemisia tridentata) | DVNHKDQLEALAGNCNWFKP GSRIIIT | Antioxidative: KD, KP, NHK Alpha-glucosidase inhibitor: EA Activating ubiquitin-mediated proteolysis: LA |
6.75 | |||
| TMV resistance protein N-like (Helianthus annuus) | DDVDHIDQLEALAGEPNWFKPGSRVII | Activating ubiquitin-mediated proteolysis: LA | 4.23 | |||
| FSRERD | 3038 | 52.8 | cyclic dof factor 1-like (Lactuca sativa) | SITSSNSRNEEFSRERDSES GVFTPKT | Hypolipidemic: EF CaMPDE inhibitor: EF |
5.00 |
| Leucine-rich repeat-containing protein (Artemisia annua) | VIGVSVGLVFIGGVMFLRHWFSRERDA | Immunostimulating: GVM Antioxidative: RHW |
9.49 | |||
| NKFSRE | Pollen receptor-like kinase 4 (Helianthus annuus) | FKRLRRLRSIFLTANKFSREIPTDA | CaMPDE inhibitor: KF |
11.83 |
* Signal-to-noise ratio on mass spectrum. ** Protein sequences matched to sequence in databases in Basic Local Alignment Search Tool (BLAST). *** Activities determined in BIOPEP-UWM database of proteins and BIOPEP-UWM database of bioactive peptides. **** Theoretical pI determined in PeptideMass ExPASy.