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(a) SERS spectrum of 0.01 µM 1,2-bis(4-pyridyl) ethylene (BPE) obtained after pre-concentration on the patterned SERS substrate with 0.7 mm spot, superimposed with the 1010 cm−1 peak for 10 μM BPE obtained with a standard non-patterned substrate and (b) a bar graph of the 1010 cm−1 peak intensity with spot diameters of 0.4, 0.7, 1.0 and 1.3 mm.
