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. 2020 May 20;13:4397–4409. doi: 10.2147/OTT.S245554

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© 2020 Chen et al.

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Overexpression of ZNF667-AS1 inhibits proliferation, migration and invasion of NPC cells. (A) RT-qPCR set to detect the expression of ZNF667-AS1 after silenced or over-expressed ZNF667-AS1 in CNE-1 cells. (B) EdU assay detected cell proliferation in each group (× 200). (C) Scratch test used to detect cell migration ability in each group (×100). (D) Transwell assay utilized to detect the number of cell invasion in each group (×100). (E) Flow cytometry adopted to detect the cell cycle entry of each group. (F) Flow cytometry used to detect the apoptosis rate of each group. *p < 0.05 vs the sh-NC group. ^#p < 0.05 vs the oe-NC group. Data are expressed as mean ± standard deviation. One-way analysis of variance is used for comparison among multiple groups.

Abbreviations: ZNF667-AS1, zinc finger protein 667-antisense RNA 1; NPC, nasopharyngeal carcinoma; RT-qPCR, reverse transcription quantitative polymerase chain reaction; EdU, 5-ethynyl-2ʹ-deoxyuridine; shRNA, short hairpin RNA; oe, overexpression; NC, negative control; DAPI, 4ʹ,6-diamidino-2-phenylindole; FL2A, focusing lens2-A; PI, propidium iodide; FITC, fluorescein isothiocyanate; G0, G0 phase; G1, G1 phase; S, S phase; G2, G2 phase; M, M phase.