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. 2020 May 20;13:4397–4409. doi: 10.2147/OTT.S245554

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© 2020 Chen et al.

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miR-1290 promotes progression of NPC cells by suppressing ABLIM1. (A) EdU assay detected cell proliferation in each group (× 200). (B) Scratch test used to detect cell migration ability in each group (× 100). (C) Transwell assay utilized to detect the number of cell invasion in each group (× 100). (D) Flow cytometry adopted to detect the cell cycle entry of each group. (E) Flow cytometry used to detect the apoptosis rate of each group. (F) Western blot analysis was conducted to detect the expression of ABLIM1, proliferation-related factor PCNA, migration-related factors N-cadherin and MMP-9, and apoptosis-related factors Bcl-2 and Bax protein in each group of cells. *p < 0.05 vs the mimic NC group. ^#p < 0.05 vs the inhibitor NC group. ^@p < 0.05 vs the miR-1290 inhibitor + sh-NC group. Data are expressed as mean ± standard deviation. One-way analysis of variance is used for comparison among multiple groups. Each experiment was run in triplicate.

Abbreviations: ABLIM1, actin-binding LIM protein 1; miR-1290, microRNA-1290; NC, negative control; DAPI, 4ʹ,6-diamidino-2-phenylindole; EdU, 5-ethynyl-2ʹ-deoxyuridine; shRNA, short hairpin RNA; FL2A, focusing lens2-A; PI, propidium iodide; FITC, fluorescein isothiocyanate; G0, G0 phase; G1, G1 phase; S, S phase; G2, G2 phase; M, M phase; GAPDH, glyceraldehyde phosphate dehydrogenase; PCNA, proliferating cell nuclear antigen; MMP, matrix metalloproteinase; Bcl2, B-cell CLL/lymphoma 2; Bax, Bcl-associated X; NPC, nasopharyngeal carcinoma; shRNA, short hairpin RNA.