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. 2020 Apr 27;25(9):2042. doi: 10.3390/molecules25092042

Figure 5.

Figure 5

Effect of genistin and genistein on protein expression and mRNA levels of AMPKα and SREBP-1c in 3T3-L1 adipocytes. 3T3-L1 cells were completely differentiated in the MDI media in the presence of 25, 50, or 100 μM genistin or genistein during the differentiation process. (A) Protein expressions of AMPKα and phosphorylated AMPKα were investigated using Western blotting. (B) Gene expression of SREBP-1c was estimated. All data were expressed as fold changes in expression relative to that in the control, untreated adipocytes (n ≥ 3, ### p < 0.001 vs. Pre (preadipocyte) group; * p < 0.05, *** p < 0.001 vs. Con (control, adipocyte) group; one-way ANOVA with the Tukey’s post-hoc test). AMPK, AMP-activated protein kinase; SREBP-1c, sterol regulatory element-binding transcription factor 1c.