Figure 10.

In vitro cell-free anti-inflammatory activity of extracts from callus cultures of S. xanthocarpum grown under different monochromatic lights conditions after 35 days of cultivation. Employed under the same experimental conditions, reference inhibitors were used as positive controls; Ibuprofen (10 µM) was used as positive control for COX-1 and COX-2 activity leading to enzyme inhibition of 31.4 ± 0.8% and 29.8 ± 1.2%, respectively; thioetheramide-PC (5 µM) was used as sPLA2 inhibitor, resulting in an inhibition of 43.7 ± 0.8%; nordihydroguaiaretic acid (100 µM) was used as 15-LOX inhibitor, leading to an inhibition of 30.6 ± 0.7%. The same volume of extraction solvent was used as blank. Data are expressed in terms of mean ± SD of at least three independent experiments. Different letters indicate significant differences between conditions (p < 0.05).