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. 2020 Apr 26;25(9):2025. doi: 10.3390/molecules25092025

Figure 5.

Figure 5

High-speed counter-current chromatography (HSCCC) chromatogram of the Fr. III. Experimental conditions: Two-phase solvent system: n-hexane-ethyl acetate-water containing 1% acetic acid (1:4:8, v/v/v); stationary phase: Upper phase; mobile phase: Lower phase; elution mode: Head to tail; flow rate: 3.0 mL/min; rotation speed: 900 rpm; separation temperature: 25 °C; detection wavelength: 254 nm; sample loading: 80 mg of the Fr. III dissolved in 5 mL of the lower phase; retention of the stationary phase: 71%. Peaks: *, isochlorogenic acid B; **, isochlorogenic acid A.