Table 1.
Partition coefficients (K) values of isochlorogenic acids A and B and the major nontarget impurity 10 in different two-phase solvent systems composed of n-hexane-ethyl acetate-water.
| No. | Solvent System (v/v) |
Partition Coefficient (K) | Separation Factor (α) | |||
|---|---|---|---|---|---|---|
| Isochlorogenic Acid B |
Isochlorogenic Acid A | Impurity 10 | Isochlorogenic Acids A and B |
Isochlorogenic Acids B and Impurity 10 | ||
| 1 | 1:3:4 | 0.21 | 0.58 | 0.30 | 2.63 | 1.43 |
| 2 | 1:3:6 | 0.36 | 1.01 | 0.35 | 2.80 | 1.03 |
| 3 | 1:4:6 | 0.63 | 2.39 | 0.58 | 3.79 | 1.08 |
| 4 | 1:4:8 | 0.74 | 3.10 | 0.66 | 4.18 | 1.12 |
| 5 | 1:4:8 * | 1.59 | 4.12 | 0.52 | 2.59 | 3.06 |
* Water containing 1% acetic acid.