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. 2020 May 26;6:38. doi: 10.1038/s41420-020-0275-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Three detection lines are on the test cassette: a control (c) line appears when serum sample flows through the card; SARS-CoV-2 antibodies presence will be indicated by a colored line in the IgG and/or IgM test line regions. If C line does not appear, the test is invalid and should be repeated. b Samples, mixed with a buffer solution containing fluorescently labeled SARS-CoV-2 recombinant proteins, form antigen–antibody conjugates. The conjugates are added to the sample well in the test card and captured by nitrocellulose-coated goat-anti-human IgM or IgG antibodies. The resulting immunocomplexes are detected by a fluorescence detector. Positive cut-off values are as follows: anti-SARS-CoV-2 IgG positive>1,2T/C; anti-SARS-CoV-2 IgM positive > 1,3T/C.