Figure 2.
Effects of tricin and enzyme-treated Zizania latifolia extract (ETZL) on cell viability and degranulation in IgE-stimulated rat basophilic leukemia (RBL-2H3) cells. (A) Cell viability; (B) β-hexosaminidase. RBL-2H3 cells were seeded in a 24-well plate (1 × 105 cells/well) overnight at 37 °C, and further incubated with anti-dinitrophenyl (DNP)-IgE (0.05 μg/mL) for 24 h. The IgE-sensitized cells were preincubated with varying concentrations of tricin (10–500 ng/mL) or ETZL (10–500 μg/mL) for 1 h, and subsequently stimulated with dinitrophenyl-human serum albumin (DNP-HSA) (0.1 μg/mL) for 4 h. All values are the mean ± S.E.M. of three independent experiments. Values of ** p < 0.01 and *** p < 0.001 were considered significantly different to the anti-DNP IgE plus DNP-HSA, unpaired Student’s t-test. # p < 0.001 compared to the control.