Skip to main content
. 2020 Apr 29;25(9):2084. doi: 10.3390/molecules25092084

Figure 7.

Figure 7

Inhibitory effect of tricin and ETZL on phosphorylation of proteins in the FcεRI signaling cascade in RBL-2H3 cells stimulated by an IgE–antigen complex. RBL-2H3 cells were seeded overnight in a 24-well plate at 37 °C; cultured cells were washed and further incubated with anti-DNP-IgE for 24 h. The cells were then incubated with varying concentrations of (A,C) tricin (10–500 ng/mL) or (B,D) ETZL (10–500 μg/mL) for 1 h, and subsequently stimulated by DNP-HSA for 4 h. All values are the mean ± S.E.M. of three independent experiments. Values of * p < 0.05, ** p < 0.01 and *** p < 0.001 were considered significantly different to the anti-DNP IgE plus DNP-HSA, unpaired Student’s t-test. # p < 0.001 compared to the control.