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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Biomaterials. 2020 Mar 4;243:119935. doi: 10.1016/j.biomaterials.2020.119935

Figure 2. Suppression of Choroidal Neovascularization (CNV) at Bruch’s Membrane Rupture Sites for 9 Weeks after Intravitreous Injection of PLGA- Acriflavine (PLGA-ACF) Microparticles (MPs).

Figure 2.

C57BL/6 mice were given an intravitreous injection of 2 μg of PLGA-ACF in one eye and 2 μg of empty PLGA MPs in the other eye. At 2, 4, 8, or 12 weeks after injection, Bruch’s membrane was ruptured by laser photocoagulation. After 1 week mice were euthanized and choroidal flat mounts were stained with FITC-Griffonia simplicifolia lectin which selectively stains vascular cells. (A) The area of CNV at Bruch’s membrane rupture sites appeared smaller in eyes injected with PLGA-ACF MP compared with those injected with empty PLGA MPs at all time points. (B) Image analysis showed a significant reduction in mean (± SEM) area of CNV in eyes injected with PLGA-ACF MPs compared with those injected with empty PLGA MPs at 2, 4, and 8 weeks after injection. N is the number of eyes evaluated in each group. (** p<0.01, * p<0.05 by unpaired t-test).