Brown Norway rats were given a suprachoroidal injection of 5 μl containing 147 μg of PLGA-ACF MPs (10 μg of ACF) in each eye. At 1, 2, 4, 8, 12, and 16 weeks after injection, three rats were euthanized, eyes removed (n=6 for each time point) and the retina and RPE/choroid was dissected and cut into superior (side of the eye that received the SC injection) and inferior halves. The level of each component of ACF, Trypaflavine (TRF) and Proflavine (PRF), were measured by LC-MS. Each point represents the mean (±SEM).