Figure 2. Severe reduction of MAIT cells in the peripheral organs in DGKζ^ΔNLS-CD4Cre mice.

Single cell suspensions of the spleen, pLNs, lung, and mononuclear cells (MNCs) of the liver from 8–10 week-old GFP-Dgkz^wt-Cd4Cre (A) or GFP-Dgkz^ΔNLS-Cd4Cre mice, and their control mice were directly stained with MR1-Tet, anti-TCRβ, CD44, CD24, and lineage antibodies. A, B. Dot plots show TCRβ and MR1-Tet straining in live gated Lin⁻ cells. Data shown are representative of at least nine experiments (A) and at least four experiments (B). C, D. MAIT cell percentages (C) and numbers (D). For both GFP-Dgkz^wt-Cd4Cre and control mice shown in C and D, data are pooled from 9 – 11 experiments (N = 11 (spleen, Lung, liver); N = 9 (LN)); For both GFP-Dgkz^ΔNLS-Cd4Cre and control mice shown in C and D, data are pooled from 4 – 7 experiments (N = 5 (spleen and lung); N = 4 (LN), N = 7 (liver)). Each circle or square represents one mouse of the indicated genotypes. *, P<0.05; **, P<0.01; ***, P<0.001 determined by pairwise Student t-test.