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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Eur J Immunol. 2019 Nov 21;50(2):192–204. doi: 10.1002/eji.201948289

Figure 7. Severe decreases of MAIT cells in the peripheral organs in DGKαζ DKO mice.

Figure 7.

Single cell suspensions of the spleen, pLNs, lung, and MNCs of the liver from 8–10-week-old Dgka−/−Dgkzf/f-CD4Cre (DKO) and DGKα+/− or WT control mice were directly stained with MR1-Tet, anti-TCRβ, CD44, CD24, CD45, and lineage antibodies. A. Dot plots show TCRβ and MR1-Tet straining in live gated Lin− cells. For lung and liver, only TCRβ+ cells were gated and shown. B. MAIT cell percentages. C. MAIT cell numbers. Data shown are representative or pooled from eight experiments (N = 8 for both control and DKO mice). Each circle and square represents one control and DKO mouse respectively. *, P<0.05; **, P<0.01; ***, P<0.001 determined by pairwise Student t-test.