Fig. 1.

IL-17 synergizes with IFNγ and TNFα to promote PD-L1 expression on MSCs. a C57BL/6 MSCs were stimulated by indicated combination of cytokines. The concentration of three cytokines was 10 ng/ml each, for this and all other figures. After 24 h, cells were harvested and prepared for quantitation of PD-L1 by real-time PCR. b C57BL/6 MSCs were treated with different combination of cytokines. Cells were collected at the indicated time points for assay of FPD-L1 mRNA by real-time PCR. c C57BL/6 MSCs were treated as in b. After 48 h, cells were harvested and the expression of PD-L1 was determined by flow cytometry. d The statistical results of three separate experiments described in c