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. 2020 May 26;10:73. doi: 10.1186/s13578-020-00431-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — IL-17 enhances the expression of PD-L1 through NO. a C57BL/6 MSCs were treated by indicated cytokines for 24 h and then collected for the measurement of NO mRNA by real-time PCR. b, c C57BL/6 MSCs were treated as in A and after 24 h (b) or indicated time points (c), supernatant were collected for the assay of NO concentration by Griess reagent. d WT MSCs (C57BL/6 MSCs) and iNOS^−/− MSCs were treated by cytokines and SMT (20 mM) for 24 h and supernatant were collected for the measurement of NO. e, f C57BL/6 MSCs were treated by cytokines with or without SMT. After 24 h, cells were collected for the assay of PD-L1 mRNA by real-time PCR. After 48 h, cells were collected for the assay of PD-L1 protein by flow cytometry. g The statistical results of three separate experiments described in f are also showed. h, i WT MSCs and iNOS^−/− MSCs were treated with cytokines. Cells were collected for the measurement of PD-L1 mRNA and protein after 24 h and 48 h, respectively. j The statistical results of three separate experiments described in i are also showed