Fig. 4.

Expression of Pfs28 in zygotes and transformed ookinetes. a Giemsa staining of retorts and ookinetes after 2nd Percoll gradient. b IFA picture of zygotes from the upper Percoll band and transformed retort and ookinete at different time points, staining with anti-Pfs28 antibody. GFP was expressed to indicate the parasite. DAPI was used to indicate the nuclei. DIC was presented to show the cell shape. The percentages of cells expressing Pfs28 at the different times analysed during the 24 h time course were presented. Scale bar = 5 µm