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. 2020 May 15;26(9-10):556–568. doi: 10.1089/ten.tea.2019.0186

FIG. 5.

FIG. 5.

Reaggregation of islet cells into spheroids with and without HUVECs. (A) Reaggregated islet cells form uniform spheroids. Scale bar, 200 μm. (B-C) Composite spheroids formed from 1000 islet cells +100/250 HUVECs are more irregular in shape and size. Scale bars, 200 μm. (D) Islet spheroids express insulin. Scale bar, 50 μm. (E) Islet spheroids embedded in vascularized chips recruit microvascular networks. Scale bar, 50 μm. (F) Increasing the ratio of HUVEC to islet cells does not increase the number of ECs in the spheroids. Most ECs are binding to the surface of a core spheroid formed almost exclusively of islet cells. Scale bar, 50 μm. (G) Composite spheroids embedded in a vascularized chip recruit microvascular networks, but we did not observe enhanced vascularization. Scale bar, 50 μm. ECs, endothelial cells.