Table 1.
Gene-specific primers used for RNAi and qPCR
| Genes | Accession No. | Primer sequence (5’ to 3’) | |
|---|---|---|---|
| Gene-specific primers used for RNAi | |||
| Pyruvate kinase, PK | AAEL014913 | Forward | GATGGTCTGGGTGTCCGATTA |
| Reverse | CTCAACCGTACAGGTCAGAGTG | ||
| Firefly luciferase, FL | U47295 | Forward | AGCACTCTGATTGACAAATACGA |
| pGL3-basic vector | Reverse | AGTTCACCGGCGTCATCGTC | |
| Gene-specific primers used for qPCR | |||
| Pyruvate kinase, PK | AAEL014913 | Forward | CTGGTGGTTGACAGTATTAGCGG |
| Reverse | CTTATCCTTTTCGGAGACGGC | ||
| Phosphoenolpyruvate carboxykinase-1, PEPCK1 | AAEL000006 | Forward | GGTCAACTGCGAGCCATCAA |
| Reverse | GAGGCGACGTTGGTAAAGAG | ||
| Phosphoglycerate dehydrogenase, PHGDH | AAEL005336 | Forward | CTGATTTCGGCTACCACGTT |
| Reverse | CTCCACCGCATTGACCAGT | ||
| NADP-dependent malic enzyme-1, NADP-ME1 | AAEL005790 | Forward | GTCATGGCTATGCGACAGGA |
| Reverse | CCAGAGATACCTCCCTTCG | ||
| Ornithine decarboxylase, ODC | AAEL007880 | Forward | CGCAGCATGAACCTAGACGT |
| Reverse | TGCTTGGCGTAGTCGAACAG | ||
| Alanine aminotransferase-1, ALT1 | AAEL028144 | Forward | CAACTGCCGGAGAAGGCAAT |
| Reverse | TGATAGGTTCCATCCTTCTGACC | ||
| Xanthine dehydrogenase-1, XDH1 | AAEL002683 | Forward | GCGATTGACATTGGACAGATCGA |
| Reverse | CCAGGAATGTCGGCGAAACC | ||
| Glutamate dehydrogenase, GDH | AAEL010464 | Forward | GGCGAGAACCTGATGTACGA |
| Reverse | GAGCAGGTGGTAGTTGGACT | ||
| Glucose-6-phosphate dehydrogenase, G6PDH | AAEL009507 | Forward | TCACAGATGCACTTTGTCCGAGC |
| Reverse | GGTCCACGAGAACCGTGCA | ||
| Ribosomal protein S7 | AAEL009496 | Forward | ACCGCCGTCTACGATGCCA |
| Reverse | ATGGTGGTCTGCTGGTTCTT |
T7 promoter sequence (5’ TAATACGACTCACTATAGGGAGA 3’) was added at 5’-end of each RNAi primer.