FIGURE 5.

HIF-1α stabilization in myeloid cells contributes to antileishmanial control in vivo. (A–D) Hif1α^Δmyel mice and littermates (controls) were infected with L. major in their hind footpads. (A) Clinical course of cutaneous L. major infection (mean ± 95% confidence interval, n ≥ 3, representative of three similar independent experiments). *p < 0.01, Student t test or Mann–Whitney U test. (B) At day 28–29 postinfection, restimulation of draining lymph node cells from L. major–infected mice with soluble Leishmania Ag was performed. IFN-γ was determined in the culture supernatants (mean + SEM, n = 9 biological samples from two independent experiments). Student t test was performed and showed no significant difference. (C) At day 29 postinfection, NOS2 protein expression was determined in lesional CD11b⁺ cells from Hif1α^Δmyel mice and littermates (control). Representative line graphs of NOS2 expression in lesional CD11b⁺ cells (left panels). Black line: NOS2 expression. Shaded area: isotype control. Geometric mean fluorescence of NOS2 (mean + SEM, n = 5–7) (right panel). A representative of two independent experiments is shown. *p < 0.05, Student t test. (D) At day 32 postinfection, L. major burden in skin lesion of infected mice was analyzed (mean + SEM, n = 8). A representative of two similar independent experiments is shown. *p < 0.05 Mann–Whitney U test.