Fig. 1.

TGF-β1 regulates the expression of adherens and tight junction proteins in cultured human renal tubule cells. Cells were cultured in low glucose control (0) ± TGF-β1 (2-10 ng/mL) for 48 h. Whole-cell expression of E-cadherin (a), N-cadherin (b), β-catenin (c), Claudin-2 (d), and ZO-1 (e) was confirmed via immunoblotting. Representative blots for each protein are shown, with expression normalized by re-probing for ɑ-tubulin as a loading control. Bars correspond to their associated lanes in the respective blot. Results were from three or more separate experiments; with key significance shown: **P < 0.01; ***P < 0.001